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trypan blue dye exclusion staining  (Thermo Fisher)


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    Thermo Fisher trypan blue dye exclusion staining
    Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
    Trypan Blue Dye Exclusion Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypan+blue+dye+exclusion+staining/pmc06368507-86-10-15
    Average 90 stars, based on 1 article reviews
    trypan blue dye exclusion staining - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells"

    Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

    Journal: Cytotechnology

    doi: 10.1007/s10616-019-00294-6

    Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
    Figure Legend Snippet: Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Techniques Used: Purification, Co-Culture Assay, Trypan Blue Exclusion Assay, Derivative Assay, Flow Cytometry

    Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
    Figure Legend Snippet: Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Techniques Used: Trypan Blue Exclusion Assay, Staining, Lactate Dehydrogenase Assay

    Related Articles

    Staining:

    Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells
    Article Snippet: When sub-confluence was reached, adherent cells were harvested after detachment with TrypLE Select solution (Lonza, Belgium) for 10 min at 37 °C and then washed in DPBS by being centrifuged at 1200× g for 10 min. .. The resulting cell pellet was recovered and the number of viable cells was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific, Belgium). ..

    Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells
    Article Snippet: Immune cell isolation, culture and activation Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation (LinfoSep, Biomedics, Madrid, Spain) and washed in Hanks’ Balanced Salt Solution (HBSS) (Lonza Europe, Verviers, Belgium). .. The number of viable PBMCs was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific). .. Following the manufacturer’s instructions, the magnetic-activated cell sorting (MACS) system technology (Miltenyi Biotec GmbH, Bergisch, Germany) was used as a method for the separation of different immune cell populations depending on their surface antigens (CD molecules).

    Article Title: Effect of standard and physiological cell culture temperatures on in vitro proliferation and differentiation of primary broiler chicken pectoralis major muscle satellite cells
    Article Snippet: .. Live cells were quantified using the CountessTM 3 Automated Cell Counter (Invitrogen, Waltham, MA) via trypan blue dye exclusion staining (Invitrogen, Waltham, MA, Cat. T10282). .. Cells were diluted in proliferation media, as described in , consisting of low-glucose DMEM with 10% chicken serum (Millipore Sigma, Burlington, MA, Cat. C5405) + 5% horse serum + 1% antibiotic/antimycotic + 0.1% gentamicin to achieve 40,000 live cells per well in triplicate on 24-well, gelatin-coated, tissue culture plates.

    Concentration Assay:

    Article Title: DMSO cryopreservation is the method of choice to preserve cells for droplet-based single-cell RNA sequencing
    Article Snippet: .. Cell concentration and cell integrity/viability were determined by trypan blue dye exclusion staining and the Countess TM cell counter (Invitrogen, Thermo Fisher Scientific, Waltham, MA) for the species mixing experiment and MDMs. .. For rat liver immune cells both parameters were estimated by the NucleoCounter NC-200 TM device (Chemometec, Allerod, Denmark; acridine orange and DAPI staining).



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    Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Journal: Cytotechnology

    Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

    doi: 10.1007/s10616-019-00294-6

    Figure Lengend Snippet: Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Article Snippet: The number of viable PBMCs was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific).

    Techniques: Purification, Co-Culture Assay, Trypan Blue Exclusion Assay, Derivative Assay, Flow Cytometry

    Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Journal: Cytotechnology

    Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

    doi: 10.1007/s10616-019-00294-6

    Figure Lengend Snippet: Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

    Article Snippet: The number of viable PBMCs was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific).

    Techniques: Trypan Blue Exclusion Assay, Staining, Lactate Dehydrogenase Assay